Anti-AKT2 (phospho S474) Antibody

Phospho-specific polyclonal reagent raised in rabbit against the serine 474 activation loop of AKT2 (RAC-PK beta / PKB beta), supplied by Antibodies.com as 50 µl of unconjugated IgG in a vial ready for direct use in western blot and immunohistochemistry workflows. S474 is the C-terminal hydrophobic motif site whose phosphorylation by mTORC2 is required for full kinase activity, so this reagent is intended for studies of PI3K/AKT signaling in insulin response, metabolic regulation, and tumor biology where AKT2-dependent phosphorylation events are being interrogated rather than total AKT2 expression. Cross-reactivity is reported for Human, Mouse, and Rat samples, and the antibody is validated on WB and IHC; researchers frequently use it alongside total AKT2 antibodies to normalize phospho-S474 signal to total protein. Store refrigerated and avoid repeated freeze-thaw cycles to preserve phospho-epitope recognition.