Anti-AKT2 (phospho S474) Antibody

Phosphorylation of AKT2 at serine 474 is a hallmark of mTORC2-dependent activation of this AGC-family kinase, marking the C-terminal hydrophobic motif that drives downstream signaling through substrates such as AS160, GSK3β, and FOXO transcription factors. This unconjugated rabbit polyclonal IgG antibody from Antibodies.com selectively recognizes the phosphorylated S474 epitope on AKT2 (also known as PKBβ), enabling Western blot and immunohistochemistry detection in human, mouse, and rat samples.

Raised in rabbit as a polyclonal reagent, the antibody is supplied as a 200 µl liquid aliquot ready for standard immunoblotting workflows and chromogenic or fluorescent IHC on formalin-fixed paraffin-embedded tissues. Investigators studying insulin signaling, glucose transport, and metabolic regulation in cancer and diabetes models commonly rely on this reagent to monitor pathway activation and confirm engagement downstream of growth factor or insulin stimulation. The unconjugated format gives flexibility to pair the antibody with secondary reagents of choice for either colorimetric or fluorescence-based readouts.

Storage and handling: Store at -20 °C in working aliquots to limit freeze-thaw cycles and protect from light. This antibody is intended for research use only.