Anti-Akt2 (Ab-474) Antibody

Manufactured by Antibodies.com, this affinity-purified rabbit polyclonal reagent targets the Akt2 protein at a region surrounding the non-phosphorylation site of Ser474, raising an antiserum against a synthetic peptide coupled to KLH and purified by epitope-specific affinity chromatography. The antibody is supplied as a 50 µL aliquot at a working concentration of 1.0 mg/mL in phosphate-buffered saline (without Mg²⁺ and Ca²⁺), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol as a cryoprotectant. Because the formulation includes sodium azide, standard precautions apply for storage of the undiluted stock, and the product is intended to detect endogenous levels of total Akt2 in Western blotting (WB) and immunohistochemistry (IHC) workflows. Suggested dilutions reported on the supplier datasheet are WB at 1:500–1:1000 and IHC at 1:50–1:100, supporting sensitive detection across a range of sample loads. The reagent is unconjugated, polyclonal, and raised in rabbit, and it has been validated for reactivity against human, mouse, and rat samples, making it a versatile choice for cross-species signaling studies of the PI3K/Akt pathway. Researchers evaluating Akt isoform expression in cell lysates or tissue sections can rely on the Antibodies.com product to deliver consistent, target-specific signal with the convenience of a ready-to-use liquid formulation.