Anti-AKT1 (phospho T450) Antibody

Phosphorylation of AKT1 at threonine 450 sits in the turn motif of the kinase domain and regulates protein stability and conformational priming prior to activation at the plasma membrane. This unconjugated rabbit polyclonal IgG reagent detects the T450-phosphorylated form of human AKT1 and is validated for western blot analysis of endogenous expression in cell lysates.

Supplied at 100 µl per vial, the antibody is unconjugated and integrates into standard immunoblotting workflows using HRP-conjugated secondary reagents. Investigators working on PI3K/AKT signaling, insulin and growth factor response, mTOR pathway crosstalk, and oncology models use this reagent to assess changes in T450 phosphorylation status following stimulation, serum starvation, or small-molecule kinase inhibitor treatment.

From Antibodies.com, this polyclonal reagent targets a post-translational modification on a kinase central to survival, proliferation, and metabolic signaling networks.