Anti-Akt1 (phospho Ser473) Antibody [D17-G]

Phosphorylation of Akt1 at Ser473 is the activating modification laid down by mTORC2, and this FITC-conjugated rabbit monoclonal clone D17-G from Antibodies.com enables direct flow cytometry detection of that phospho-epitope without a secondary antibody. The reagent supports single-cell analysis in human samples, resolving the active, signaling-competent fraction of Akt1 in lymphocyte, tumor, and primary cell populations. Supplied as 100T in liquid format with IgG isotype, the antibody fits growth factor response studies, survival signaling work, and metabolic regulation research. Because the fluorophore is conjugated directly to the clone, the staining protocol is shorter and the background from cross-reactive secondaries is reduced, which is useful when looking at rare phospho-positive subsets within a mixed population. Reactivity is annotated for human Akt1, and the liquid product form allows direct dilution into staining buffer for intracellular flow cytometry protocols after standard fixation and permeabilization.