Anti-AKT1 (phospho S473) Antibody

From Antibodies.com, this rabbit polyclonal antibody recognises AKT1 when phosphorylated at Ser473. It is supplied as a liquid, unconjugated and of IgG isotype, and is affinity-purified. The catalogue lists applications including Western blotting and immunohistochemistry, with reactivity reported against human, mouse and rat samples, while the manufacturer's validation data is for Western blotting on human and rat samples. The recommended Western blot dilution range is 1:500–1:1,000.

The target protein has a molecular weight of 60 kDa. The immunogen is a synthetic phosphorylated peptide around S473 of human Akt1 (NP_005154.2), with the sequence QFSYS. The formulation is Phosphate Buffered Saline at pH 7.3, containing 50% glycerol and 0.02% sodium azide. The antibody is shipped at 4°C and should be stored at –20°C upon receipt, with freeze/thaw cycles avoided.