Anti-AKT1 (E40) Antibody

Produced in rabbit as an unconjugated polyclonal reagent, this Antibodies.com antibody recognizes AKT1 (RAC-alpha serine/threonine-protein kinase), a central effector of PI3K-mediated survival and growth signaling. Supplied at 1 mg/ml in phosphate buffered saline (PBS) with 0.05% sodium azide at approximately pH 7.2, the 100 µl volume supports multiple western blot and immunohistochemistry experiments on human, mouse, and rat samples. The polyclonal reagent shows reactivity across these three species, enabling cross-species comparative studies in cancer biology, insulin signaling, and cardiovascular research. AKT1 phosphorylation at Thr308 and Ser473 serves as a key biomarker for pathway activation, and this antibody is commonly used to assess total AKT1 expression alongside phospho-specific antibodies as a normalization control. The PBS-based formulation with sodium azide preservative is suitable for direct incubation on membranes or tissue sections following standard protocols. Refrigerate to preserve antibody activity across the working shelf life.