Anti-Akt1 Antibody [V19-T]

Targeted to Akt1, a serine/threonine kinase central to PI3K/Akt signaling that governs cell survival, proliferation, and glucose metabolism, this FITC-conjugated rabbit monoclonal antibody from Antibodies.com enables direct fluorescent detection in flow cytometry. The V19-T clone provides isoform-level specificity for Akt1 in human samples, distinguishing it from the related Akt2 and Akt3 paralogs that have overlapping but distinct roles in growth factor and insulin signaling.

Direct FITC conjugation eliminates the need for a fluorophore-labeled secondary antibody, which reduces nonspecific binding, shortens staining protocols, and simplifies multi-color panel design. The reagent is supplied as a liquid in a 100T vial and is validated for intracellular flow cytometry on fixed and permeabilized cells, making it useful for phospho-flow analysis of pathway activation in activated lymphocytes, cancer cell lines, and primary human tissue samples.

Investigators studying mTOR signaling, PTEN loss, insulin resistance, or oncogenic transformation frequently profile Akt1 as a marker of pathway activity. The antibody is of the IgG isotype and reacts with human Akt1. To preserve FITC fluorescence, store refrigerated and protect from light; avoid repeated freeze-thaw cycles and aliquot on first use for long-term storage.