Anti-Akt1 Antibody

Raised in rabbit and affinity-purified from antiserum, this unconjugated polyclonal primary antibody from Antibodies.com is supplied as a 50 µl liquid aliquot for research-grade detection of endogenous Akt1 (also known as RAC-alpha serine/threonine-protein kinase / PKB-alpha). Validated for Western Blot (WB) and ELISA workflows, the reagent shows reactivity against human, mouse and rat samples and is provided at 1 mg/ml in a PBS buffer containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. The host species is rabbit, clonality is polyclonal with an IgG isotype, the conjugate is unconjugated (free for downstream HRP or fluorescent labelling), and the recommended long-term storage is at −20 °C for up to one year, with freeze-thaw cycles avoided to preserve activity. Antibodies.com lists this reagent under their Primary Antibodies catalogue as an anti-Akt1 pAb suitable for PI3K/Akt signalling studies, cell lysate Western blotting and quantitative ELISA; for Western blot a working dilution range of 1:500–1:1,000 is typically used, while ELISA is performed at 1:100,000–1:200,000 depending on the coating antigen. The product is intended for research use only and is not validated for diagnostic or therapeutic applications.