Anti-Akt1 Antibody

From Antibodies.com, this rabbit polyclonal reagent is raised against Akt1, a serine/threonine protein kinase that operates downstream of PI3K and governs cell survival, proliferation, glucose metabolism, and angiogenesis. The antibody is unconjugated and supplied as a 100 µl aliquot, ready for direct labelling or use in standard detection workflows.

Validated across three independent applications, the antibody performs reliably in Western blot, immunohistochemistry, and ELISA. Researchers deploy it to track total Akt1 expression levels and to investigate perturbations of the PI3K/Akt/mTOR signaling axis in cancer biology, insulin resistance studies, and cardiovascular research models.

Cross-reactivity has been confirmed in human, mouse, and rat samples, enabling consistent results across cell-line panels and primary tissue lysates from multiple species. As a polyclonal reagent, the antibody recognizes multiple epitopes across Akt1, which supports robust signal even when a single binding site is masked by post-translational modification or sample processing.

The target itself, Akt1 (also called PKBα), is an AGC-family kinase recruited to the plasma membrane by PIP3, where phosphoinositide-dependent kinase 1 activates it through phosphorylation at Thr308 and Ser473. Detecting changes in Akt1 abundance and modification state therefore gives researchers a quantitative readout of upstream pathway activity.

This Antibodies.com product is intended for research use only and supports laboratories running routine immunoblotting of phospho-Akt panels, IHC surveys of tumor microarrays, and ELISA-based quantification of Akt1 in treated cell lysates.