Anti-AKT1 (Ab-450) Antibody

This polyclonal antibody from Antibodies.com is raised in rabbit against AKT1 phosphorylated at threonine 450 (Thr450), a regulatory autophosphorylation site within the turn motif of the AKT1 kinase domain. AKT1, also called protein kinase B alpha (PKBα), sits at the core of the PI3K/AKT signaling cascade and regulates cell growth, glucose metabolism, survival, and proliferation.

Supplied as 100 µL of unconjugated reagent, the antibody is intended for western blot analysis of AKT pathway activation in cell and tissue lysates, immunohistochemistry on fixed sections, and immunofluorescence staining of cultured cells. Cross-reactivity has been validated against mouse and rat AKT1 alongside the human target, supporting translational work in standard laboratory models.

For working use, store at -20°C in small aliquots to limit freeze-thaw cycles and protect from light. Researchers in oncology, metabolic disease, and intracellular signal transduction routinely deploy phospho-Thr450 AKT1 antibodies to monitor pathway activation states.