Anti-AKT (phospho-Y326) Antibody

This rabbit polyclonal antibody from Antibodies.com is raised against AKT phosphorylated at tyrosine 326, a post-translational modification on the AKT kinase family implicated in PI3K-AKT-mTOR signal transduction. The reagent is supplied at 1 mg/ml in phosphate buffered saline (PBS) containing 0.05% sodium azide at approximately pH 7.2, and is packaged in a 100 µl vial format that fits standard immunohistochemistry workflows on formalin-fixed, paraffin-embedded tissue sections.

The unconjugated antibody is validated for IHC application and shows reactivity against human, mouse, and rat samples, supporting cross-species studies of phospho-AKT signaling in tumor biology, neurodegeneration, and metabolic disease research. Refrigerated storage is recommended to preserve activity, and the sodium azide preservative permits brief handling at room temperature during dilution and staining procedures. Aliquotting on first use limits repeated freeze-thaw cycles of the stock solution.

Because the reagent is polyclonal, it is more likely to detect the phospho-Y326 epitope even when neighboring residues vary slightly between samples, an advantage in tissue sections where epitope accessibility is often partial. Researchers investigating insulin signaling, growth factor responses, or downstream mTOR pathway activation will find this a focused tool for mapping site-specific AKT phosphorylation in their target tissues.