Anti-AKT (phospho-T450) Antibody

Affinity-purified from rabbit serum and unconjugated, this Antibodies.com polyclonal reagent selectively detects AKT phosphorylated at threonine 450 (pT450), a turn-motif residue whose modification supports kinase folding and stability rather than acute activation. Supplied as 100 µL at approximately 1 mg/ml in phosphate buffered saline (pH 7.2) containing 15 mM sodium azide, the reagent binds the phosphorylated epitope in human, mouse, and rat samples.

T450 sits in the turn motif of AKT, distinct from the canonical activating phospho-sites threonine 308 and serine 473, so this reagent is most often paired with anti-pT308 and anti-pS473 antibodies when dissecting PI3K/AKT/mTOR pathway activity. Researchers apply it in western blot analysis of total AKT levels and in immunohistochemistry on fixed tissue sections, with common use in studies of insulin signaling, cancer cell lines carrying PI3K or PTEN alterations, and downstream readouts of mTORC2-dependent regulation. For daily use, store refrigerated; aliquot the 100 µL volume and freeze at -20 °C for longer-term storage, avoiding repeated freeze-thaw cycles.