Anti-AKT (phospho-Ser473) Antibody

This recombinant rabbit monoclonal antibody from Antibodies.com is raised against AKT phosphorylated at Serine 473, a modification widely used as a marker of PI3K/AKT pathway activation. The phospho-specific reagent is supplied at 50 µl, unconjugated, and validated for western blot, immunocytochemistry/immunofluorescence, and immunohistochemistry workflows. It reacts with human and mouse AKT, supporting cross-species studies in cell signaling research. The antibody is formulated in TBS (pH 7.4) with 1% BSA, 40% glycerol, and 0.05% sodium azide as preservative, supplied as a recombinant clone for reliable lot-to-lot performance. AKT, also known as Protein Kinase B, is a serine/threonine kinase whose activation drives downstream mTOR signaling, glucose metabolism, cell survival, and proliferation, so phosphorylation at Ser473 is routinely monitored in lysates and tissue sections. Researchers studying insulin receptor signaling, growth factor responses, oncogenic transformation, or responses to kinase inhibitors use this antibody to confirm pathway engagement. As a rabbit monoclonal reagent it offers the affinity advantage of a rabbit host combined with the specificity of a single epitope, helping reduce background in phospho-proteins that often give weaker signal than total-protein targets.