Anti-AKT (phospho-Ser473) Antibody

This recombinant rabbit monoclonal antibody from Antibodies.com specifically recognizes AKT only when phosphorylated at Serine 473, a key regulatory site in the PI3K/AKT/mTOR signaling cascade. The reagent is supplied as 100 µl of unconjugated antibody in a TBS (pH 7.4) buffer containing 1% BSA, 40% glycerol, and 0.05% sodium azide as preservative.

Validated applications include western blot (WB), immunocytochemistry/immunofluorescence (ICC/IF), and immunohistochemistry (IHC), with reactivity confirmed against human and mouse samples. Researchers deploy this clone to monitor pathway activation downstream of growth factor, insulin, and cytokine receptors, and to investigate AKT involvement in oncology, metabolic disease, neuroscience, and cardiovascular biology. Phosphorylation at Ser473 is largely driven by mTORC2 and serves as a principal readout of full AKT kinase activity, complementing the Thr308 site.

To preserve activity, aliquot upon arrival to minimize freeze-thaw cycles and store frozen at -20°C. The unconjugated format allows flexible pairing with secondary reagents of the researcher's choice, including HRP-, fluorophore-, or biotin-conjugated detection systems. This reagent is intended for research use only and is not approved for diagnostic or therapeutic applications.