Anti-AKT (D302) Antibody

Useful in studies of PI3K-AKT-mTOR signaling, glucose metabolism, cell survival, and proliferation, this unconjugated rabbit polyclonal primary antibody from Antibodies.com is raised against the D302 epitope region of AKT (also known as Protein Kinase B / PKB), a serine/threonine kinase. The reagent is supplied as 50 µl of Ig at 1 mg/ml in phosphate buffered saline (pH 7.2) with 0.05% sodium azide as preservative, and is validated for Western blot and immunohistochemistry (IHC) on human, mouse, and rat samples. Researchers investigating oncogenic signaling, insulin response, apoptosis pathways, and downstream phosphorylation of GSK3β, mTOR, and BAD substrates apply AKT antibodies to cell lysates and tissue sections. The polyclonal format tolerates minor epitope variation, and the unconjugated conjugate leaves detection chemistry flexible for HRP, Alexa Fluor, or biotinylated secondary reagents.