Anti-Akt Antibody

Manufactured by Antibodies.com, this primary antibody is a rabbit polyclonal reagent raised against Akt and supplied in a 50 µl volume as an unconjugated, affinity-purified preparation at approximately 1 mg/mL in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. The polyclonal antibody detects endogenous levels of total Akt protein and is validated for Western blot (WB), immunohistochemistry (IHC) and ELISA workflows, making it suitable for routine detection of Akt signalling across a broad range of cell and tissue samples. The host species is rabbit, the clonality is polyclonal (IgG), and the conjugate is unconjugated, allowing flexible pairing with secondary antibodies and custom labelling strategies. Cross-reactivity has been confirmed in human, mouse and rat samples, supporting translational and comparative studies in mammalian models. The immunogen corresponds to a peptide derived from the human Akt sequence, enabling recognition of the native protein in endogenous contexts. For best stability and activity the reagent should be stored at –20 °C in working aliquots and subjected to minimal freeze/thaw cycles; the glycerol-containing buffer helps prevent ice crystal damage during cold storage. As a research-use-only reagent, it is intended for laboratory applications and is not cleared for diagnostic or therapeutic use. Researchers seeking a robust, broadly reactive anti-Akt polyclonal antibody for standard WB, IHC and ELISA assays will find this Antibodies.com reagent a reliable choice within the 50 µl size format.