Anti-Akt Antibody

Akt, also known as Protein Kinase B (PKB), is a serine/threonine kinase central to the PI3K/Akt/mTOR signaling cascade governing cell survival, proliferation, glucose metabolism, and protein synthesis. This rabbit polyclonal reagent from Antibodies.com targets the Akt protein and is supplied as an unconjugated 100 µl preparation for western blot, immunohistochemistry, and ELISA workflows.

Validated reactivity spans human, mouse, and rat samples, and the polyclonal backbone recognizes multiple epitopes across the Akt sequence for detection in tissue sections and whole-cell lysates. Investigators commonly apply this reagent to track activation through phosphorylation at residues such as Ser473 and Thr308 in tumor models, insulin signaling studies, and neurodegeneration research.

The unconjugated format accommodates secondary reporters conjugated to HRP, biotin, or fluorophores, enabling chromogenic western blot imaging and fluorescence immunohistochemistry on fixed sections. Each vial supports repeated experimental runs across multiple blots or staining protocols.