Anti-Akt Antibody

Akt, also known as Protein Kinase B (PKB), is a 56-62 kDa serine/threonine kinase central to the PI3K/Akt signalling pathway, where it governs cell survival, growth, proliferation, metabolism, and apoptosis. This polyclonal reagent from Antibodies.com targets total Akt and is supplied as 100 µl of unconjugated antiserum raised in rabbit, suitable for Western blot, immunohistochemistry, and ELISA workflows. Reactivity has been verified in Human, Mouse, and Rat samples, supporting translational studies ranging from rodent disease models to human tissue analysis. The polyclonal composition enables broad recognition of epitopes shared across the three Akt isoforms (Akt1, Akt2, Akt3), which is useful when researchers need to detect total Akt signal rather than a single isoform. Aliquot upon receipt and store at -20 °C to preserve antibody integrity across multiple freeze-thaw cycles; for active use, keep a working aliquot refrigerated at 4 °C. The unconjugated format allows pairing with HRP, biotin, or fluorescently-labelled secondary antibodies of the researcher's choice. Each vial is shipped from Antibodies.com with lot-validated QC data and application-specific recommendations to support reproducible downstream assays.