Anti-Akt Antibody

Raised in rabbit and affinity-purified by epitope-specific immunogen chromatography, this polyclonal reagent from Antibodies.com is designed to detect endogenous levels of Akt protein across a broad panel of experimental workflows. The immunogen is a synthesized peptide derived from human Akt around the non-phosphorylation site of S473, and the antibody cross-reacts with human, mouse, and rat samples, making it suitable for translational research projects that compare signalling between species. It is supplied as an unconjugated liquid in PBS containing 50% glycerol, 0.5% BSA, and 0.02% sodium azide, at a working concentration of 1 mg/mL, and is offered here in a 50 µl pack size. Validated applications include ELISA, Western blot (WB), immunocytochemistry/immunofluorescence (ICC/IF), and immunohistochemistry (IHC), so the same lot can be used for quantitative antigen detection on membranes, subcellular localisation in cultured cells, and tissue-section staining. For routine bench use, vials are shipped at 4°C and should be aliquoted upon arrival and stored at –20°C, with repeated freeze/thaw cycles avoided to preserve activity. As a research-use-only reagent, this Antibodies.com polyclonal offers a flexible, multi-application option for laboratories studying the PI3K/Akt pathway in oncology, metabolism, and neuroscience models.