Anti-Akt Antibody

This rabbit polyclonal antibody from Antibodies.com is raised against Akt (Protein Kinase B), a serine/threonine kinase central to the PI3K/Akt/mTOR signaling cascade that governs cell survival, proliferation, glucose metabolism, and apoptosis.

Validated for Western blot, immunohistochemistry, and ELISA applications, the unconjugated reagent recognizes the Akt family across human, mouse, and rat samples. Because Akt sits at a convergence point for growth factor, insulin, and cytokine signaling, it is a frequently cited marker in oncology, diabetes, and neuroscience research, where investigators probe pathway activation, phosphorylation status, and crosstalk with downstream effectors such as mTOR and GSK-3β.

Supplied as 100 µl of polyclonal antiserum raised in rabbit, the antibody supports detection of endogenous Akt expression in cell lysates, tissue sections, and prepared antigens. It pairs with standard HRP- or fluorophore-conjugated secondary antibodies for chromogenic or fluorescent detection workflows, and can be applied in studies of isoform-specific signaling (Akt1, Akt2, Akt3) when combined with appropriate experimental controls.