Anti-Akt Antibody

Manufactured by Antibodies.com, this affinity-purified Rabbit polyclonal reagent is raised against a synthetic peptide derived from the human Akt sequence flanking the non-phosphorylation site at S473, and the polyclonal antibody specifically detects endogenous levels of total Akt protein. The unconjugated antibody is supplied as a liquid at 1 mg/mL in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, and is offered in a 50 µL pack size that is shipped at 4 °C with instructions to aliquot and store at –20 °C, avoiding repeated freeze/thaw cycles. Validation data published on the Antibodies.com product page support its use in Western blot, immunohistochemistry, immunocytochemistry/immunofluorescence and ELISA workflows, and the reagent has been confirmed to cross-react with Human, Mouse and Rat samples, making it suitable for both cell-line and tissue-based studies. The host species is Rabbit, the clonality is polyclonal and the conjugate is unconjugated, allowing end-users to couple it directly to their preferred reporter or to use it as a primary layer in custom ELISA, WB and IHC protocols. The product is intended for research use only and is not validated for diagnostic or therapeutic applications, with recommended dilutions of WB 1:500–1:1000, IHC 1:50–1:100 and ELISA 1:20000 as detailed on the supplier datasheet, and is shipped from St. Louis, MO with a typical lead time of 6–9 business days plus freight.