Anti-Akt (Ab-473) Antibody

A rabbit polyclonal reagent from Antibodies.com specifically recognizes Akt when phosphorylated at threonine 473 (Thr473), the activating site targeted by mTORC2 within the PI3K/Akt signaling cascade. This 100 µL unconjugated antibody is validated for Western blot and immunohistochemistry on human and mouse specimens, supporting pathway analysis, cancer biology, and metabolic research. Investigators use it to track downstream signaling in response to growth factors, insulin stimulation, or oncogenic cues, to quantify phosphorylation changes during small-molecule inhibitor treatment, and to localize active Akt across archived tissue sections. The polyclonal format provides broad recognition of the phosphorylated Akt pool, which is useful when total Akt expression varies across samples, treatment arms, or disease models.