Anti-AKR1C3 Antibody

Generated in rabbit and affinity purified, this unconjugated polyclonal reagent from Antibodies.com recognizes AKR1C3 (Aldo-Keto Reductase Family 1 Member C3, also called HSD17B5), a cytosolic NADPH-dependent oxidoreductase that reduces aldehydes, ketones, and steroid precursors across androgen, estrogen, and prostaglandin pathways. The antibody is supplied at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol as a 100 µl aliquot, ready for direct use in western blot and immunohistochemistry workflows on samples prepared from human, mouse, and rat tissues.

AKR1C3 is widely investigated in hormone-driven cancers, particularly castration-resistant prostate cancer and certain breast and endometrial tumors, where expression is frequently elevated relative to matched normal tissue. Researchers studying prostaglandin F synthase activity, type 5 17β-hydroxysteroid dehydrogenase function, or xenobiotic carbonyl metabolism commonly use this target to track pathway activity by western blot of cell lysates or by immunohistochemistry on formalin-fixed, paraffin-embedded sections, both of which are validated applications for this antibody.

Store the vial at -20 °C in the glycerol-containing buffer to preserve activity across multiple freeze-thaw cycles, and aliquot to working volumes to minimize repeated handling of the stock. The unconjugated format leaves the antibody available for downstream labeling with HRP, biotin, or fluorophore conjugates chosen to match a preferred detection chemistry.