Anti-AKR1B10 Antibody

Raised in rabbit against a recombinant fragment spanning amino acids 1–286 of human AKR1B10, this polyclonal reagent from Antibodies.com detects the aldo-keto reductase family 1 member B10 protein in cell lysates, paraffin sections, and cultured cells. The antibody was generated by antigen-affinity purification and is supplied as a 100 µL vial of IgG in 0.1 M Tris-buffered saline at pH 7.0 containing 10% glycerol, with 0.01% thimerosal added as a preservative. Validation data on the Antibodies.com product page show a band of the expected size by Western blot using 30 µg of MOLT-4 or Raji whole-cell lysate at a 1:1000 dilution, nuclear and cytoplasmic staining on paraffin-embedded OVCAR3 xenograft sections at 1:500 by immunohistochemistry, and clear cytoplasmic signal on paraformaldehyde-fixed HeLa cells at 1:200 by immunofluorescence. Recommended handling is to aliquot upon arrival and store the working vial refrigerated at 4 °C for short-term use, with longer-term storage at −20 °C while avoiding repeated freeze-thaw cycles. The unconjugated format allows pairing with any user-selected secondary antibody for chromogenic or fluorescence detection, reactivity has been confirmed on human samples, and the product is restricted to research use only.