Anti-AKR1B1 Antibody

Manufactured by Antibodies.com, this research-grade reagent is a mouse monoclonal antibody raised against recombinant full-length human AKR1B1 (aldose reductase). It is supplied as a 50 µl aliquot of purified immunoglobulin in a buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl, 0.2% sodium azide, and 50% glycerol, and is presented in an unconjugated format for maximum flexibility in downstream labelling. The clone was affinity-purified from mouse ascites by epitope-specific affinity chromatography and reaches approximately 95% purity by SDS-PAGE. Validated applications include Western blotting (WB) and immunocytochemistry (ICC), where it produces a clean band at the predicted molecular weight of approximately 36 kDa. The antibody specifically detects endogenous levels of AKR1B1 and does not cross-react with related family members, making it a reliable tool for studies of the polyol pathway, diabetic complications, and related oxidative-stress research in human cells and tissues. Reactivity is confirmed for human samples. For best stability, the product should be stored according to the standard Antibodies.com recommendations for purified monoclonal antibodies in glycerol-containing Tris buffer, and orders are typically dispatched from Cambridge (UK) within 5–8 business days.