Anti-AKR1A1 Antibody

This unconjugated polyclonal primary antibody from Antibodies.com targets AKR1A1, a cytosolic aldo-keto reductase that reduces a broad aldehyde substrate spectrum, participates in hepatic xenobiotic metabolism, and supports osmotic and redox homeostasis. Raised in rabbit, the reagent is supplied as 100 µl aliquot for immediate use in standard immunodetection workflows.

Reactivity has been confirmed on Human, Mouse, and Rat samples, supporting detection of the approximately 36 kDa AKR1A1 monomer in whole-cell lysates, tissue homogenates, and recombinant protein preparations. The polyclonal pool recognizes multiple epitopes across the enzyme, improving recovery of both full-length and partially processed forms during immunoblotting.

For Western blot, recommended working dilutions fall between 1:500 and 1:2,000 depending on antigen abundance, with HRP-conjugated anti-rabbit secondary antibodies and chemiluminescent substrates producing a discrete band after SDS-PAGE separation and transfer to nitrocellulose or PVDF membranes. The unconjugated format also adapts readily to ELISA workflows where user-preferred reporter enzymes can be coupled directly to the primary reagent.