Anti-AKAP8 Antibody

A-kinase anchoring protein 8 (AKAP8), also known as AKAP95, is a nuclear scaffold that tethers protein kinase A (PKA) at chromatin and contributes to mitotic chromosome condensation, DNA replication, and pre-mRNA splicing. The Antibodies.com rabbit polyclonal antibody is raised against this target and supplied as an unconjugated 100 µl aliquot for western blot, immunohistochemistry, and immunofluorescence workflows. Cross-reactivity is reported against human, mouse, and rat samples, supporting comparative studies of nuclear signaling and PKA compartmentalization across model systems. Polyclonal reactivity broadens epitope coverage, aiding detection of full-length AKAP8 and potential isoforms in cell lysates and fixed tissue sections. Researchers characterizing cell-cycle progression, chromatin-associated signaling, and splicing factor recruitment commonly apply this reagent to localize endogenous AKAP8 by immunofluorescence, confirm expression by western blot, and map tissue distribution through IHC. Brief centrifugation before use and refrigeration of working aliquots help preserve antibody activity over repeated experiments.