Anti-AKAP10 Antibody

An affinity-purified polyclonal reagent raised in rabbit against AKAP10, a mitochondrial scaffolding protein that anchors type I and type II regulatory subunits of cAMP-dependent protein kinase A (PKA) at defined subcellular compartments. Antibodies.com supplies this Primary Antibodies format reagent as 100 µL of unconjugated antisera, validated for Western blot and immunohistochemistry workflows on human samples.

The polyclonal host response targets multiple epitopes on AKAP10, which can improve signal recovery in fixed tissue sections where single epitopes may be masked by aldehyde crosslinking. Researchers investigating mitochondrial signalling, cAMP compartmentation, and cardiac or neuronal PKA targeting rely on this reagent to probe endogenous AKAP10 expression in cell lysates and archived specimens. Because the antibody is unconjugated, it can be paired with a secondary detection reagent of the user's choice, including HRP, biotin, or fluorophore conjugates, depending on the assay readout.

Aliquot upon first use and protect from unnecessary freeze-thaw cycles; refrigerate short-term to preserve binding activity across multiple experiments. For IHC, an antigen retrieval step is typically required on paraffin-embedded material to expose masked epitopes, while Western blot workflows on mitochondrial-enriched fractions give the cleanest signal-to-noise for this target.