Anti-AKAP 95 Antibody

Reagent Profile

A-kinase anchoring protein 95 (AKAP 95, encoded by the AKAP8 gene) is a nuclear scaffold that tethers cAMP-dependent protein kinase A (PKA) regulatory subunits to chromatin and the nuclear matrix. It plays structural and regulatory roles during interphase and cell division, supporting chromatin condensation at mitosis, S-phase DNA replication initiation, and meiotic resumption in oocytes. Altered AKAP 95 expression has been reported in several solid tumor contexts, which has increased its profile as a marker of interest in cancer biology research.

This Rabbit polyclonal reagent from Antibodies.com is raised against AKAP 95 and supplied as an unconjugated antibody in a 100 µl volume. It is validated for western blot, immunohistochemistry, immunofluorescence, and ELISA workflows, and shows reactivity against Human, Mouse, and Rat samples. The polyclonal format provides epitope diversity for detection of the full-length approximately 95 kDa nuclear protein across denatured and native preparations.

Recommended storage is at -20°C in working aliquots to limit freeze-thaw cycles, with short-term use at 4°C as needed. The unconjugated format allows flexible pairing with HRP, fluorophore, or biotin secondary reagents depending on the chosen detection platform. For immunohistochemistry, antigen retrieval and permeabilization are typically required because the antigen localizes to the nuclear compartment.