Anti-AK4 Antibody - Identical to Abcam (ab196792)

Raised in rabbit against adenylate kinase 4 (AK4), this unconjugated polyclonal IgG reagent targets the mitochondrial GTP:AMP phosphotransferase also known as AK3L1. The 50 µl vial from Antibodies.com recognizes conserved epitopes shared across human, mouse, and rat orthologues, making the reagent useful for comparative studies of mitochondrial nucleotide metabolism in cell lines, primary cultures, and tissue specimens. Validated uses span western blot (WB), immunohistochemistry (IHC), immunofluorescence (IF), and immunoprecipitation (IP), with working dilutions of 1:500 to 1:3000 for WB, 1:100 to 1:250 for IHC on paraffin sections, and 1:100 to 1:200 for IF imaging.

AK4 sits at a metabolic junction, coupling adenine nucleotide interconversion with the cellular response to hypoxia and oxidative stress, so laboratories commonly deploy this antibody to profile mitochondrial adaptations in tumor models. Published studies link altered AK4 expression to proliferation and survival phenotypes in lung adenocarcinoma, breast carcinoma, and serous ovarian cancer, where immunostaining and pull-down experiments with the AK3L1 reagent help discriminate cancer-cell metabolic states from adjacent normal tissue. Because the antibody is unconjugated, it pairs freely with HRP, fluorophore, or biotin secondary reagents of the user's choice, supporting chromogenic, fluorescence, and chemiluminescence detection on the same primary.

Store the vial at -20 °C and avoid repeated freeze-thaw cycles by working from small aliquots held at 4 °C during active use. The polyclonal format confers multi-epitope recognition, which improves tolerance to minor sample-preparation differences and preserves signal across RIPA, NP-40, and mild detergent lysates. For research use only.