Anti-AK1 Antibody

This rabbit polyclonal antibody from Antibodies.com targets adenylate kinase 1 (AK1), the cytosolic isoform that catalyzes reversible phosphoryl transfer between ATP and AMP to maintain cellular nucleotide balance. In striated muscle, brain, and erythrocytes, AK1 supports rapid ATP regeneration, and its expression is commonly examined in metabolic, mitochondrial, and erythrocyte maturation studies.

Supplied as 100 µL of unconjugated antiserum, the reagent is validated for western blot, immunohistochemistry, and immunofluorescence workflows. It detects endogenous AK1 in human, mouse, and rat samples, enabling cross-species comparisons in basic research on cellular energetics, myocyte biology, and adenine nucleotide interconversion.

On western blots of whole-cell lysates, the antibody recognizes the approximately 22 kDa AK1 monomer, allowing researchers to probe changes in expression under hypoxic, ischemic, or exercise-mimetic conditions. Immunohistochemistry on fixed tissue sections can be used to map AK1 distribution across skeletal and cardiac muscle fibers, while immunofluorescence supports co-localization studies with mitochondrial markers in cultured cells. Investigators studying AK1 deficiency-related hemolytic anemia and inherited metabolic disorders also rely on this reagent for patient-derived sample characterization.

The polyclonal format offers tolerance for minor sequence variation, supporting detection across multiple species when paired with appropriate HRP- or fluorophore-conjugated secondary antibodies. Researchers should consult the lot-specific data sheet shipped with each Antibodies.com order for recommended working dilutions across individual applications.