Anti-AIFM1 Antibody

This rabbit polyclonal antibody targets AIFM1, also known as Apoptosis-Inducing Factor (AIF) or PDCD8, a mitochondrial flavoprotein essential for caspase-independent programmed cell death. The unconjugated reagent is supplied by Antibodies.com as a 100 µl aliquot and is validated for Western blot, immunohistochemistry, and immunofluorescence workflows. Cross-reactivity has been confirmed against human, mouse, and rat samples, supporting translational projects that compare AIFM1 expression across model organisms and patient-derived material.

AIFM1 functions as an FAD-dependent NADH oxidase in healthy mitochondria, where it contributes to complex I assembly and respiratory chain stability. Upon apoptotic signalling, the protein is cleaved and translocates to the nucleus, driving large-scale chromatin condensation and DNA fragmentation independent of caspase activation. Laboratories studying neurodegeneration, ischemic injury, and inherited mitochondrial disease use this primary antibody to monitor AIFM1 processing and subcellular redistribution during cell death pathways, as well as to probe the maturation of the apoptogenic fragment that escapes the intermembrane space after a loss of mitochondrial membrane potential.

For best results, store refrigerated in working aliquots to limit repeated freeze-thaw cycles of the stock solution and preserve antibody activity over time. The polyclonal format recognises multiple epitopes on the target, supporting western blot detection of the mature ~67 kDa mitochondrial isoform and the truncated ~57 kDa apoptogenic fragment that emerges during programmed cell death.