Anti-AHI1 Antibody

The Antibodies.com Anti-AHI1 polyclonal reagent is raised in rabbit and supplied as an unconjugated IgG preparation in a 200 µl vial, intended for Western blot detection of human AHI1, the protein also known as Jouberin. The antibody recognizes the endogenous target in cell and tissue lysates, supporting researchers working on Joubert syndrome type 3, ciliogenesis, and the neurodevelopmental pathways in which AHI1 participates at the basal body of primary cilia.

Aliquot upon arrival and store frozen to preserve reactivity through multiple freeze-thaw cycles, avoiding repeated freeze-thaw of the working stock. The polyclonal format increases the likelihood of recognizing multiple epitopes on AHI1, which is useful when probing truncated isoforms or patient-derived variants in disease models. Beyond standard Western blot workflows on human samples, the reagent is suited to studies of retinal and cerebellar development, where AHI1 expression patterns inform hypotheses about the cerebellar-ocular-renal phenotypes seen in Joubert syndrome carriers and the protein's broader role in schizophrenia susceptibility loci.

Practical notes: pair with an HRP-conjugated anti-rabbit secondary, image via chemiluminescence, and load 20-40 µg of total protein per lane for clear band resolution near the predicted molecular weight of AHI1.