Anti-AGR3 Antibody

This rabbit polyclonal reagent from Antibodies.com targets AGR3 (Anterior Gradient 3), an endoplasmic reticulum-resident protein linked to estrogen receptor signaling and investigated extensively in breast, ovarian, pancreatic, and other epithelial cancers. The unconjugated antibody is supplied as 100 µl of liquid and is validated for Western blot, immunohistochemistry, and immunofluorescence workflows on human samples.

AGR3 belongs to the protein disulfide isomerase-like family (also annotated as PDIA18) and is upregulated in several solid tumors, where it has been explored as a biomarker of disease progression and metastatic potential. Researchers use polyclonal anti-AGR3 antibodies to probe ER stress responses, characterize circulating tumor cells, and study anterior gradient homologs in normal versus transformed human epithelium.

Storage recommendations for this Antibodies.com reagent typically involve aliquoting upon arrival and storing at -20 °C in a frost-free freezer to preserve activity across multiple freeze-thaw cycles. For short-term use, the antibody can be kept refrigerated at 4 °C. Working dilutions should be optimized per application; for immunohistochemistry on formalin-fixed paraffin-embedded sections, antigen retrieval in citrate buffer pH 6.0 is commonly advised, and Western blot signals from AGR3-expressing cell lysates are typically detected in the 18–22 kDa range.

For research use only, this reagent is intended for laboratory investigation of cancer biology, biomarker discovery, and basic study of AGR family function in vitro.