Anti-AGPAT1 Antibody

Raised in rabbit against the AGPAT1 enzyme, this unconjugated polyclonal antibody recognizes human 1-acylglycerol-3-phosphate O-acyltransferase 1, the membrane-bound acyltransferase that converts lysophosphatidic acid to phosphatidic acid during de novo glycerophospholipid biosynthesis. Antibodies.com supplies the reagent at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol, shipped as a 100 µl aliquot that can be stored at -20°C and aliquoted to limit freeze-thaw degradation of the glycerol-stabilized stock. The antibody has been validated for Western blot detection of AGPAT1 expression in human cell and tissue lysates and for immunohistochemistry on formalin-fixed paraffin-embedded sections, supporting research into hepatic lipid metabolism, triacylglycerol and phospholipid homeostasis, membrane biogenesis, and the recessive AGPAT1 loss-of-function variants associated with congenital generalized lipodystrophy type 1. Provided as a single-use vial, the polyclonal format offers recognition of multiple epitopes on the target, an advantage when probing post-translational modifications or splice variants of the lipid biosynthetic enzyme.