Anti-AFP Antibody

This Rabbit polyclonal antibody from Antibodies.com targets Alpha-Fetoprotein (AFP), a 70 kDa glycoprotein that serves as a key biomarker in clinical research. AFP expression is most active during fetal development, produced primarily by the yolk sac and fetal liver, but it also reappears at elevated levels in adults with certain malignancies, making anti-AFP antibodies valuable tools in oncology research and tumor marker studies.

Validated for Western blot (WB) and ELISA workflows, the unconjugated antibody is supplied as 100 µl of polyclonal antiserum raised in rabbit, with reactivity confirmed against human AFP. Researchers use it to detect AFP in cell lysates, tissue extracts, and serum-derived samples from hepatocellular carcinoma studies, germ cell tumor investigations, and prenatal screening research.

Because the antibody is unconjugated, it can be paired with a labeled secondary reagent to fit the chosen detection platform. Working dilutions should be titrated for each individual assay, and the reagent should be handled according to standard cold-chain laboratory practice to preserve its activity across repeated freeze-thaw cycles.