Anti-ADSS Antibody

Adenylosuccinate synthetase, the GTP-dependent ligase that converts inosine monophosphate to adenylosuccinate in the de novo purine nucleotide biosynthesis pathway feeding AMP, is the target of this Anti-ADSS polyclonal reagent from Antibodies.com. Mammalian cells express two isoforms — ADSS1, the basic muscle-enriched isozyme, and ADSS2, the acidic isozyme — and biallelic ADSS1 loss-of-function variants are an established cause of MLASA syndrome (concurrent myopathy, lactic acidosis, and sideroblastic anemia). That link has placed ADSS on the radar in studies of mitochondrial muscle metabolism, nucleotide pool maintenance under metabolic stress, and the cell-intrinsic control of purine supply in proliferating myoblasts.

Shipped as 100 µl of an unconjugated preparation at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol as cryoprotectant, this Antibodies.com polyclonal reagent has been validated for western blot analysis of human, mouse, and rat lysates and for immunohistochemistry on tissue sections. The unconjugated format keeps the antibody flexible for HRP- or fluorophore-conjugated secondary detection on immunoblots, on fixed tissue sections, and in immunofluorescence workflows. Working aliquots are recommended to limit freeze-thaw cycling of the glycerol-stabilized stock, with the 0.1% sodium azide suppressing microbial contamination during extended refrigerated handling.