Anti-ADP-ribosylation factor 4 Antibody

This rabbit polyclonal antibody targets ADP-ribosylation factor 4 (ARF4), an approximately 20 kDa GTP-binding protein of the ARF/Arl Ras superfamily that controls vesicular trafficking between the trans-Golgi network and endosomal compartments. ARF4 participates in cargo sorting at the trans-Golgi, retrograde transport pathways, lipid droplet biogenesis, and ciliogenesis, and altered expression has been documented in several tumor types.

Supplied by Antibodies.com as a 100 µg pack in a single vial, the reagent is unconjugated and validated for Western blot and immunohistochemistry on human samples. The polyclonal format provides multi-epitope coverage of the endogenous GTPase, supporting detection in cell lysates, tissue sections, and subcellular fractionation experiments. The antibody is raised in rabbit and intended for research use only; users commonly pair it with appropriate HRP- or fluorophore-conjugated secondary antibodies for chemiluminescent or fluorescence detection.

Best practices include brief centrifugation before opening and aliquoting to limit freeze-thaw cycles. The product supports studies of Golgi organization, membrane traffic, cilia biology, and ARF4 expression profiling in cancer and other human tissues.