Anti-ADORA3 Antibody

ADORA3, also known as the adenosine A3 receptor, is a G protein-coupled receptor implicated in inflammatory signaling, ischemic cardioprotection, and mast cell activation. This rabbit polyclonal IgG reagent from Antibodies.com is raised against the human ADORA3 protein and validated for Western blot and immunohistochemistry workflows on human, mouse, and rat samples. Supplied unconjugated in a 100 µl volume, it allows flexible labeling and detection choices depending on the secondary reagent selected for a given experiment.

Researchers commonly deploy this antibody to map ADORA3 distribution in tissue sections, to track receptor expression changes in disease models, and to probe signaling crosstalk downstream of adenosine binding. Because ADORA3 couples to Gi/Gq pathways, its detection can support studies of cyclic AMP modulation, calcium release, and downstream kinase activation in primary cells and cell lines. The polyclonal format provides recognition of multiple epitopes on the receptor, which is useful when working with samples where post-translational modifications or sequence variation may affect a single epitope.

For Western blot, the antibody is suited to detecting ADORA3 bands in membrane-enriched fractions from tissues such as heart, lung, and spleen. In immunohistochemistry, it can be applied to formalin-fixed paraffin-embedded sections to localize receptor expression at the cellular level. Store refrigerated and avoid repeated freeze-thaw cycles to preserve performance across multiple experiments.