Anti-ADH1C Antibody

Raised in rabbit against ADH1C (alcohol dehydrogenase 1C, class I gamma subunit), this unconjugated polyclonal IgG reagent from Antibodies.com detects the ~39 kDa human enzyme that catalyzes the NAD-dependent oxidation of ethanol to acetaldehyde. The 100 µl vial is supplied as an affinity-purified antibody in a buffered aqueous solution suitable for Western blot and immunohistochemistry workflows on human, mouse, and rat specimens. Recommended starting dilutions are 1:500–1:2000 for immunoblotting of cell or tissue lysates and 1:50–1:200 for staining of paraffin-embedded tissue sections. Because the reagent is unconjugated, laboratories can pair it with HRP-, biotin-, or fluorophore-conjugated secondary antibodies matched to their preferred detection chemistry, including multiplexed imaging panels. For continuous use, store at -20°C and avoid repeated freeze-thaw cycles; aliquoting upon first thaw preserves activity across long projects. The polyclonal format recognizes multiple epitopes on ADH1C, which can be advantageous for tracking full-length protein expression in comparative studies of hepatic, gastric, and other ADH1C-expressing tissues across the three reactive species. Researchers should validate performance with appropriate positive controls (ADH1C-expressing human liver lysates) and negative controls, and optimize fixation, antigen retrieval, blocking, and incubation conditions for their specific protocol.