Anti-ADH1B Antibody

Raised against human alcohol dehydrogenase 1B (ADH1B), this rabbit polyclonal antibody from Antibodies.com is supplied as 100 µl of unconjugated reagent validated for Western blot (WB) and immunohistochemistry (IHC) workflows on human samples. ADH1B is a zinc-containing, cytosolic enzyme of the medium-chain dehydrogenase/reductase family that catalyzes the NAD+-dependent oxidation of ethanol to acetaldehyde, with predominant expression in liver and additional transcript presence in kidney, lung, and gastrointestinal tract; it also contributes to retinol metabolism, making it a focal target in studies of hepatic biology, alcohol-use research, and pharmacogenetic investigations of polymorphic variants such as ADH1B*2 (Arg47His, rs1229984) that accelerate acetaldehyde generation. The polyclonal format recognizes multiple epitopes across the ~40 kDa ADH1B subunit, which is helpful when probing denatured protein on PVDF or nitrocellulose membranes and for visualizing ADH1B-positive hepatocytes within fixed tissue sections. Unconjugated formulation gives laboratories flexibility to pair the reagent with HRP, biotin, Alexa Fluor, or other reporter-conjugated secondary antibodies for chromogenic, chemiluminescent, or fluorescence-based detection. Researchers working on ethanol clearance rates, hepatic gene expression profiling, or immunohistochemistry-based tissue surveys of human liver and gastrointestinal samples will find this reagent a versatile addition to their antibody panel.