Anti-ADD1 (phospho S726) Antibody

Rabbit polyclonal IgG antibody from Antibodies.com, supplied as an unconjugated reagent in a 100 µl vial, raised against Alpha-Adducin phosphorylated at serine 726. ADD1 (Adducin Alpha) is a cytoskeletal protein that caps the fast-growing ends of actin filaments and promotes the association of spectrin with actin at the membrane skeleton. Phosphorylation at S726 by protein kinase C reduces adducin's affinity for the spectrin–actin complex, a regulatory step tied to cytoskeletal remodeling downstream of calcium- and diacylglycerol-dependent signaling pathways.

Validated for Western blot, immunohistochemistry, and immunofluorescence on human, mouse, and rat samples, the reagent supports detection of the phosphorylated epitope in cell lysates and fixed tissue sections. Work on cytoskeletal dynamics, signal-dependent remodeling of the cortical skeleton, renal sodium handling, and hypertension-associated cell biology relies on phospho-site-specific adducin antibodies to monitor kinase activity in stimulated cells and primary tissue. The polyclonal format offers multi-epitope recognition of the S726 phospho-target, which can improve signal over monoclonal alternatives in weakly expressing systems.

Handling recommendations include refrigerating the vial on arrival and aliquoting on first use to limit freeze-thaw cycles; protect from light during longer-term storage. The product is supplied as a single-use vial for research use only and is intended for laboratory investigation of phospho-regulated cytoskeletal control.