Anti-ADD1 (Ab-726) Antibody

Manufactured by Antibodies.com, this rabbit polyclonal antibody is raised against ADD1 (alpha-adducin) and recognizes an epitope surrounding amino acids 724–728 (T-P-S-F-L) of the human protein, enabling detection of the endogenous total ADD1 protein in cell and tissue lysates. Supplied as a 50 µL aliquot at 1.0 mg/mL in phosphate-buffered saline (pH 7.4) containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol, the reagent is unconjugated and of the IgG isotype, leaving flexibility for downstream labeling and detection chemistries. Validated applications include Western blot (WB), immunohistochemistry (IHC), and immunocytochemistry/immunofluorescence (ICC/IF), with cross-reactivity confirmed in human, mouse, and rat samples. The antibody was generated by immunizing rabbits with a synthetic peptide conjugated to KLH and purified by affinity chromatography on an epitope-specific peptide column, yielding high specificity for total ADD1 regardless of phosphorylation state. For stability, the vial is shipped at 4 °C and should be aliquoted upon receipt and stored at –20 °C, with repeated freeze/thaw cycles avoided to preserve activity for continuous use. Synonymous names associated with the target include ADDA, Alpha-adducin, and Erythrocyte adducin subunit alpha, reflecting the protein's role as a membrane–cytoskeleton-associated component that binds calmodulin and promotes assembly of the spectrin–actin network. Intended for research use only, this affinity-purified polyclonal reagent from Antibodies.com provides a versatile tool for laboratories investigating adducin biology across multiple model systems.