Anti-ADAM1B Antibody [#158]

Clone #158 was raised against a recombinant fragment encompassing the disintegrin-like domain of murine ADAM1B, the region responsible for heterodimer pairing with ADAM2 (fertilin alpha) on the sperm membrane. This reagent recognizes both the full-length precursor and the smaller mature species that arise after prodomain cleavage during late spermiogenesis, so a single western blot run on 20-40 ug of testis lysate per lane resolves both biosynthetic and processed forms of fertilin beta. In immunofluorescence on fixed cryosections, the antibody decorates round and elongating spermatids within the seminiferous epithelium, while immunohistochemistry on paraffin-embedded murine reproductive tissue produces consistent labeling of post-meiotic germ cells. For optimal handling, prepare single-use aliquots upon first thaw, store at -20 °C for short-term use, and transfer working stocks to -80 °C for extended storage; repeated freeze-thaw cycles should be avoided to preserve binding activity. The unconjugated IgG formulation from Antibodies.com is supplied as a liquid at 100 µg per vial and is intended for research use only, supporting custom fluorophore or enzyme conjugation workflows in laboratories studying gamete recognition.