Anti-ADAM10 Antibody

This rabbit polyclonal antibody targets ADAM10 (A Disintegrin And Metalloproteinase 10), a transmembrane metalloprotease widely studied for its role in ectodomain shedding of cell-surface proteins, including amyloid precursor protein in the non-amyloidogenic α-secretase pathway and ligands in Notch signaling. Researchers investigating Alzheimer's disease, tumor invasion, neuroinflammation, and TNF-α cytokine release use this reagent to map expression and localization in tissue and cell lysates.

Validated applications include Western blot and immunohistochemistry across human and mouse samples. The unconjugated IgG is supplied as 100 µl at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol, giving direct compatibility with HRP-, biotin-, or fluorophore-based secondary detection systems. Aliquot upon receipt and store at -20°C; the glycerol cryoprotectant preserves activity through routine freeze-thaw cycles.

From Antibodies.com, this polyclonal reagent offers epitope coverage across multiple regions of ADAM10, useful for detecting both full-length and processed forms of the protease. The combination of broad species reactivity and dual-format validation supports experiments ranging from cell-line screening to paraffin-embedded tissue analysis.