Anti-ACY1 Antibody

ACY1 (Aminoacylase 1) is a zinc-binding cytosolic metalloenzyme that hydrolyzes N-acyl-L-amino acids into free amino acids, supporting intracellular amino acid recycling and the detoxification of N-acetyl intermediates. This Rabbit polyclonal antibody from Antibodies.com was raised against recombinant human ACY1 and is supplied as 100 µl of unconjugated antisera for use in Western blot and immunohistochemistry workflows.

Reactivity and clonality

Polyclonal host: Rabbit. Clonality: Polyclonal. Conjugate: Unconjugated. Demonstrated reactivity with Human and Mouse samples. Heterogeneous recognition of multiple epitopes on ACY1 typically yields consistent signal across denatured lysates (WB) and fixed, paraffin-embedded tissue sections (IHC).

Validated applications

• Western blot detection of endogenous ACY1, observed near the expected molecular weight of approximately 46 kDa, in cell and tissue lysates.
• Immunohistochemistry on formalin-fixed, paraffin-embedded sections for tissue-level distribution studies.

Biological context

ACY1 is broadly expressed in liver and kidney, and altered levels have been reported in renal cell carcinoma, hepatocellular carcinoma, and certain leukemia cell lines. Investigators working on N-terminal protein processing, aminoacylase deficiency, or tumor metabolism can apply this reagent to interrogate expression patterns in their models.

Handling

Shipped as a liquid polyclonal preparation. Upon receipt, aliquot into working volumes and store at -20 °C to limit freeze-thaw cycles. Protect from light. Research use only.