Anti-ACTN2 Antibody

Alpha-actinin 2 (ACTN2) is a sarcomeric Z-disc protein expressed in cardiac and skeletal muscle, where it cross-links actin thin filaments and anchors signaling complexes at the sarcomere boundary. The Antibodies.com rabbit polyclonal antibody to ACTN2 supports research into striated muscle biology, sarcomere architecture, and the cardiomyopathy-linked gene variants that disrupt Z-disc organization.

Validated for Western blot and immunohistochemistry, this unconjugated reagent reacts with both human and mouse samples. It is widely used to visualize Z-disc integrity in cardiac tissue sections, probe alpha-actinin 2 levels in cardiomyocyte lysates, and characterize sarcomere remodeling in models of hypertrophic and dilated cardiomyopathy. Researchers studying ACTN2 mutations, myofibrillar myopathies, and exercise-induced muscle adaptation also rely on this reagent for Z-line localization studies.

Supplied as 100 µl of polyclonal antisera raised in rabbit, the antibody is unconjugated and can be paired with user-selected secondary detection reagents for immunofluorescence, chromogenic IHC, or standard Western blot protocols. The 100 µl volume supports multiple staining runs and provides flexibility for both small pilot experiments and larger screening panels.