Anti-Actived-Caspase-9 p35 (D315) Antibody

The p35 subunit generated by caspase-9 cleavage at Asp315 marks commitment to the intrinsic apoptotic pathway, and this polyclonal reagent from Antibodies.com is raised in rabbit against that specific neo-epitope region. Supplied as a 100 µl vial of unconjugated IgG at 1 mg/ml in phosphate buffered saline containing 0.05% sodium azide at approximately pH 7.2, the antibody is validated for Western blot and immunohistochemistry on human, mouse, and rat samples, making it a practical tool for comparing caspase-9 activation across cultured cells, primary tissues, and animal disease models.

Because the reagent discriminates the processed fragment from full-length pro-caspase-9, researchers can use it to track the kinetics of mitochondrial-driven apoptosis following treatment with cytotoxic agents, growth factor withdrawal, or DNA damage, and to localize cleaved caspase-9 within affected regions of fixed tissue sections. Aliquot upon first use, store refrigerated, and protect from repeated freeze-thaw cycles of the stock solution to preserve antibody activity over its working shelf life.