Anti-Active Caspase-3 Antibody

Caspase-3 is a central executioner protease in the apoptotic cascade, and selectively detecting its cleaved, catalytically active fragment — rather than the inactive zymogen — is essential for measuring ongoing programmed cell death. This recombinant rabbit monoclonal antibody from Antibodies.com binds the activated form of human Caspase-3, enabling researchers to resolve the p17 and p12 cleavage products by Western blot and to localize apoptotic cells by immunocytochemistry, immunofluorescence, and immunohistochemistry on fixed tissue sections. The reagent is supplied as 100 µL of unconjugated antibody in a stabilizing buffer composed of TBS at pH 7.4 with 1% BSA, 40% glycerol, and 0.05% sodium azide as preservative; for best results, aliquot upon arrival and store refrigerated, avoiding repeated freeze-thaw cycles. Validated reactivity with human samples makes this antibody well suited to apoptosis studies in cancer biology, neurodegeneration models, developmental biology, and toxicology screens.